【文献标题】The response of performance in grower and finisher pigs to diets formulated to different tryptophan to lysine ratios

【作者】Liu J.B，Yan H.L，Cao S.C，et.al

【作者单位】西南科技大学（Southwest University of Science and Technology）

【文献中引用产品】

猪皮质醇（Cortisol）ELISA试剂盒

【关键词】Broken-line，Pig，Requirement，Standardized ileal digestible，Tryptophan to lysine ratio

【DOI】doi.org/10.1016/j.livsci.2019.01.016

【影响因子(IF)】2.50

【出版期刊】《Livestock Science》

【产品原文引用】

The feed ingredient and diet samples were analyzed following AOAC (2006) procedures for CP, ether extract, crude fiber, and Ca and P concentration. The AA profile of ingredients and diets were analyzed using HPLC (Hitachi L-8800 AA Analyzer; Hitachi, Tokyo, Japan) after one of 3 hydrolysis procedures (AOAC, 2006). Most of the AA were determined after acid hydrolysis in 6 N HCl at 110 °C for 24 h. For the determination of S-containing AA, the oxidation process was performed before the acid hydrolysis using performic acid. The analysis of Trp was performed after alkaline hydrolysis. The SUN concentration was determined using a biochemical analytical instrument (Byer Diagnostics Manufacturing Ltd., Dublin, Ireland). The plasma cortisol was determined by using an Enzyme-Linked Immuno-Sorbent Assay kit for porcine cortisoldetermination (Hengyuan Bio-technology, Shanghai,China). The intra-assay coefficient of variation was 10%.

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