【文献标题】Programming changes in GLUT1 mediated the accumulation of AGEs and matrix degradation in the articular cartilage of female adult rats after prenatal caffeine exposure

【作者】Qing-xian Li，Lin-long Wang，et.al

【作者单位】武汉大学中南医院（Zhongnan Hospital of Wuhan University）

【文献中引用产品】

试剂-藏红o试剂(CAS. No. 477-73-6)

试剂-固绿FCF试剂(CAS. No. 2353-45-9)

【关键词】Prenatal caffeine exposure，Glucocorticoid，Glucose transporter 1，Glucose metabolism，Articular cartilage，Cartilage development

【DOI】doi.org/10.1016/j.phrs.2019.104555

【影响因子(IF)】9.90

【出版期刊】《Pharmacological Research》

【产品原文引用】

Caffeine (CAS #58-08-2, > 99% purity) was purchased from SigmaAldrich Co., Ltd. (St. Louis, MO, USA). Isoflurane was purchased from Baxter Healthcare Co. (Deerfield, IL, USA). Safranin O (CAS No. 477-73-6) and Fast Green (CAS No. 2353-45-9) were obtained from Hengyuan Biotech (Shanghai, China). A TRIzol reagent (SKU: R6830-02) was purchased from Omega Bio-Tek (Doraville, GA, USA). Reverse transcription and quantitative PCR kits were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). Oligonucleotide primers for the gene used for analysis were synthesized by Sangon Biotech Co., Ltd.(Shanghai, China). RIPA lysis buffer and a BCA Protein Assay kit were purchased from Beyotime Institute of Biotechnology (Haimen, China).Western Bright ECL HRP substrate was purchased from Advansta (Menlo Park, CA, USA). Fetal bovine serums were supplied by Gibco (St.Louis, MO, USA). Recombinant Murine IGF1 (250-19) was purchased from PeproTech, Inc. (Rocky Hill, NJ, USA). Advanced glycation end products (AGE-BSA, 2221-BSA) and BSA (2221-10) were purchased from BioVision (Milpitas, CA, USA). Low molecular weight heparin (LMWH) was purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO,USA). Collagenase II was obtained from Invitrogen (Carlsbad, CA, USA).Polyclonal antibody information is as follows: glyceraldehyde 3-phosphatedehydrogenase (GAPDH) (No. AC033), anti-β-actin (No.AB_2768234), α1 chain of type II collagen gene (COL2A1) (No.ABP51023), aggrecan (ACAN) (No. bs-1223R), GLUT1 (No. 21829-1-AP), AGEs (No. bs-1158R), ADAM metallopeptidase with thrombospondin type 1 motif 5 (ADAMTS-5) (No. ab41037), matrix metallopeptidase 13 (MMP13) (No. ab39012), IGF1 (No. sc-9013), and acetylhistone 3 lysine 9 (H3K9) (No. A7255). Secondary antibody information is as follows: Cy3 conjugated goat anti-rabbit IgG (H + L) (No.B100801) and Cy3 conjugated goat anti-rabbit IgG (H + L) (No. AS-1111). The other chemicals and agents were analytical grade.

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