【文献标题】Study on antitumor molecular mechanism of Alisols based on p53DNA

【作者】Fei Xu，Cai Lu， Qinan Wu，et.al

【作者单位】南京中医药大学（Nanjing University of Chinese Medicine）

【文献中引用产品】

试剂-台盼蓝/台盼兰

【关键词】Alisols，Antitumor activity，Molecular mechanism

【DOI】doi.org/10.1016/j.ijbiomac.2018.05.158

【影响因子(IF)】6.90

【出版期刊】《International Journal of Biological Macromolecules》

【产品原文引用】

Reagents: DMEM medium and phosphate buffer saline (PBS)

(HyClone, USA); penicillin and fetal bovine serum (FBS) (Gibco, USA);Methyl thiazolyl tetrazolium (MTT) (Beyotime, China); Dimethyl sulfoxide (DMSO) and trypsin (Amresco, USA); Trypan-blue (Hengyuan Biological Technology, China); Paclitaxel standard (Sigma-Aldrich, USA);human breast cancer cell line (MCF-7) (ASTRI Cell Resource Center,China); Single-stranded DNA (Sangon Biotech, China):P1 promoter region of p53 gene (ss1: 5′-CCTCCTCCCCAACTCC-3′, ss2: 3′-GGAGGAGGGGT TGAGG-5′); Alisol A and alisol B (Mansite Biotechnology,China) (structures shown in Fig. 1 [3]); Ethidium bromide (EB)(Macklin, China); Acetonitrile (LC, Merck, USA); Trihydroxymethyl aminomethane (Tris, BIO LINK, USA); Tris and hydrochloric acid (HCl)were used to prepare Tris-HCl buffer (0.50 mol·L−1), pH 7.4; All other chemicals used were of analytical grade and experimental water for ultrapure water.

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