【文献标题】Sphk1 participates in malignant progression of breast cancer by regulating epithelial-mesenchymal transition and stem cell characteristics

【作者】Zhijie Chen, Bingxiong Liu

【作者单位】汉川市人民医院（Hanchuan People’s Hospital）

【文献中引用产品】

霍乱毒素 CAS号：9012-63-9

【关键词】Breast cancer，Sphingosine kinase 1，Epithelial-mesenchymal transition，Stem cell，Metastasis

【DOI】doi.org/10.1016/j.tice.2020.101380

【影响因子(IF)】2.90

【出版期刊】《Tissue and Cell》

【产品原文引用】

Cell and culture

Human breast epithelial cells (MCF-10A, CRL-10317) and BC cell lines MCF-7 (CRL-3435), T47D (HTB-133), SKBR3 (HTB-30), MDA-MB-231 (HTB-26), BT-474 (HTB-20) were purchased from American Tissue Culture Collection. The cells, except MDA-MB-23 cells, were cultured in DMEM (12491015, ThermoFisher, USA) containing 10 % FBS (30067334, ThermoFisher, USA), 1% penicillin/streptomycin (15140163,ThermoFisher, USA) at 37 °C in 5% CO2. MCF-10A cell line was cultured with additional 100 ng/mL cholera toxin (Hengyuan,China, hyswbio on the basis of other cell culture condition. After culture for one day, the original medium was discarded, and the cells were washed 1–2 times by PBS. Next, 5 mL fresh DMEM medium was added and further incubated with the cells in a CO2 incubator. When the confluence reached 80%–90%, the cells were subcultured. MDA-MB-231 cells were cultured in Leibovitz’s L15 medium (11415064, ThermoFisher, USA) without CO2。

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